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DEVELOPMENT OF ELECTRONIC DEVICES FOR ELECTROPORATION
desire for more accurate research of electroporation also requires
new directions in development of electronic devices. The EP1
electroporator, which we developed in 1998, generates monopolar pulses with
amplitudes up to 600V, while APEX, developed in 1999, generates
bipolar pulses up to 600 Vpp. In 2001 we also developed a device
(GMS7.1) that allows for simultaneous connection of up to seven
electrodes. From 2000 to 2003 we participated in the development of
a clinical electroporator (CliniporatorTM) within the EU
project QLK3-1999-00484. Currently we are working on a device that
will generate arbitrary signals up to 2000 Vpp. As an upgrade of
CliniporatorTM, we are also developing an ECG
synchronizer, which will minimize the risk of heart defibrillation.
Fundamental understanding of electroporation can be gained from
experiments on lipid bilayers, the simplest model of the membrane.
For this we developed a system that delivers arbitrary signals up to
1.5 V and measures currents as low as several nA.
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Puc M, Corovic S, Flisar K, Petkovsek M, Nastran J, Miklavcic D.
Techniques of signal generation required for
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Bioelectrochemistry 64: 113-124, 2004. [PDF]